The limitation of Optical microscope is not associated with the technology of the microscope rather than it is linked with the property of light. Whereas, nucleus microscope can be utilized for higher technology along with a thin and non-living specimens. The Atomic Force Microscopes are accountable for this predicament since they can assist researchers in stealing a look into the nano scale world. There is merely one negative aspect of these microscopes
Before digging into the downside, let us see how an Atomic Force Microscope works. Foremost, allow me to present some insight about the working of this microscope. This Atomic Force Microscope (AFM) is a form of scanning probe microscope (SPM). They are utilized in the measurement of height, friction, magnetism and other local properties. The SPM raster scans probe on an area of the sample in order to get a proper image. In the meantime, it also assesses the local attributes of that image.
Then the nanowire probe is exceptionally thin and its thinness can be imagined by the fact, since it is 500 times tenuous than a hair. The function of this nano wire is to stimulate on the surface of the sample for the measurement. The sole drawback of this microscope probe is that it cannot be used along with the light of a laser. The reason behind this is that the energy of laser over power the sensitive measurement.
Frequent switching of the laser light is the only way out of this problem. Speedily take measurements when the laser is off and as soon as the nano probe arrive back at the room temperature switch it on again. Repeating the procedure will provide you with a correct value.
The Atomic Microscopes have thousand times higher resolution as compared to the Optical Microscopes
